超微粉碎稻谷糠壳发酵生产燃料丁醇的研究

Application of Superfine Grinding Rice Hull Acid Hydrolysis in Acetone-Butanol-Ethanol(ABE) Fermentation

摘要: 以超微粉碎后的稻谷糠壳为原料, 以丙酮丁醇梭菌为发酵菌株, 考察了不同硫酸浓度和酸水解时间对发酵生产丙酮、丁醇、乙醇(ABE)总溶剂浓度的影响。同时进一步研究其酸水解液不同脱毒方式, 添加碳源和营养物质对ABE总溶剂产量的影响。结果表明: 体积分数为1.5%的硫酸121℃酸水解30 min, 利用Ca(OH)₂过中和酸水解液脱毒, 发酵78 h最终ABE总溶剂量为14.62 g/L, 较未处理提高30.10%, 残糖质量浓度为8.46 g/L, 较未处理下降47.8%;超微粉碎后稻谷糠壳酸水解液中的营养成分已能满足丙酮丁醇梭菌发酵生产ABE溶剂所需, 添加葡萄糖碳源和其他营养成分并不能显著提高ABE总溶剂产量和缩短发酵时间。

Abstract: The process for producing ABE by fermentation from Superfine grinding rice hull acid hydrolysis was studied. Based on the ABE concentration, fermentation time, reduce sugar concentration and initial sugar concentration, different detoxification methods and acid hydrolysis rate were investigated. The optimized conditions for the production were 1.5 %(V/V) of Sulfuric acid hydrolysis for 30 min at 121 ℃, then neutralization by Ca(OH)₂ for detoxification. After 78h, 14.62 %(V/V) of ABE was produced, and remained sugar was only 8.46 g/L. Since it provided the necessary carbon source and nutrition for ABE fermentation, there was no need to supplement these additionally.