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1673-159X

CN 51-1686/N

Fe(Ⅲ)-过氧化氢-亚硝酸盐催化BSA蛋白硝化的影响研究

Study on Protein Nitration of BSA Induced by Fe(Ⅲ)-H2O2-NO2- System

    摘要
  • 摘要: 采用分光光度法研究了不同pH Hepes缓冲液中Fe(Ⅲ)-过氧化氢-亚硝酸盐催化牛血清白蛋白(BSA)发生蛋白质硝化的情况。实验结果表明: 在Hepes缓冲液中, EDTA-Fe(Ⅲ)及柠檬酸铁均能够催化过氧化氢-亚硝酸盐引起BSA的硝化。在EDTA-Fe(Ⅲ)-过氧化氢-亚硝酸盐体系中, BSA的硝化程度随pH的升高而依次增强, 亚硝酸盐含量随pH值的升高而降低, 说明碱性环境有利于EDTA-Fe(Ⅲ)-过氧化氢-亚硝酸盐催化BSA发生蛋白质硝化; 而在柠檬酸铁-过氧化氢-亚硝酸盐体系中, BSA的硝化程度随pH的升高而依次降低, 亚硝酸盐含量随pH值的升高而升高, 说明酸性环境有利于柠檬酸铁-过氧化氢-亚硝酸盐催化BSA发生蛋白质硝化。进一步研究表明: EDTA-Fe(Ⅲ)催化过氧化氢–亚硝酸盐发生蛋白质硝化作用强于柠檬酸铁。

     

    Abstract: In this paper, the effect of Hepes buffers and pH on Fe(Ⅲ)-H2O2-NO2-system induced bovine serum albumin(BSA) nitration was studied by using spectrophotometry.The result showed that BSA could be nitrated by EDTA-Fe(Ⅲ) and Ferric Citrate in Hepes buffers.In EDTA-Fe(Ⅲ)-H2O2-NO2-system, protein nitration was increased with the increase in pH, concentration of NaNO2 was decreased with the increase in pH, the results showed that in EDTA-Fe(Ⅲ)-H2O2-NO2-system, alkaline was conducive to the conduct of protein nitration.While in Ferric Citrate-H2O2-NO2-system, protein nitration was decreased with the increase in pH, concentration of NaNO2 was increased with the increase in pH, the results showed that in Ferric Citrate-H2O2-NO2-system, acid was conducive to the conduct of protein nitration.In addition, further study indicated that protein nitration induced by EDTA-Fe(III)-H2O2-NO2-was stronger than that of Ferric Citrate-H2O2-NO2-.

     

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