Abstract: The extraction of hop polyphenol and the scavenging function for DPPH radical are researched in this paper. The optimal processing condition was attained by single-factor test and RSM. When acetone concentration is 64%, the extraction temperature is 52 degrees and the ratio of material to solvent is 1 to 24. Extracted for an hour in this optimized condition, the polyphenol maximum value is 49.25 mg/g. At the same time, the scavenging effect for DPPH radical is tested.The result indicates that the scavenging rate EC50(Half elimination concentration) of polyphenol material in hop for DPPH free radical is 28.913μg/mL.